PE Conjugated Anti-Human CD329 Monoclonal Antibody (Clone: K8)
Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD329 (K8) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
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| Amount : | 100 tests |
| Isotype : | Mouse IgG1 kappa |
| Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
CD329 is a type I transmembrane glycoprotein of siglec family, which contains intracellular ITIM motif, and a SLAM-like motif, that acts as a docking site for SAP. CD329 can regulate TCR signaling by recruitment of SHP-1, which results in down-regulation of TCR-based gene transcription. It is expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells, weaker expression is detectable in some B cells, NK cells, and T cells.
Specificity: The mouse monoclonal antibody K8 recognizes an extracellular epitope of CD239, a type I transmembrane glycoprotein expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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