PE Conjugated Anti-Human CD270 (Clone: CW10)
Figure 1: Separation of SK-MEL-30 cells stained using anti-human CD270 (CW10) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from SK-MEL-30 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/ml, same as CD270 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of SK-MEL-30 cell suspension.
Roll over image to zoom in
Shipping Info:
For estimated delivery dates, please contact us at [email protected]
Amount : | 100 tests |
Isotype : | Mouse IgG1 kappa |
Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Content : | Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
CD270 is a type I transmembrane protein of the TNFR superfamily, which is expressed on resting T cells, monocytes, and immature dendritic cells. Its ligands, CD258 and CD272, differ in effect on CD270 signaling. Whereas binding to CD258 provides a costimulatory signal, binding to CD272 gives to the cell an inhibitory signal. CD270 also is recognized by herpes simplex glycoprotein D. CD258-CD270 interaction and signaling is implicated in macrophage-derived foam cell-mediated development of atherosclerotic lesions.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
There are currently no product reviews
|