PE Conjugated Anti-Human C3aR Mab (Clone: HC3aRZ8)
Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C3aR (HC3aRZ8) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
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| Amount : | 100 tests |
| Isotype : | Mouse IgG2b |
| Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Formulation : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Specificity : The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.
C3aR is a 7TM transmembrane protein associated with G proteins, and serves as a receptor for C3a complement fragment. It is expressed mainly on neutrophils, mast cells, basophils, eosinophils, dendritic cells, monocytes, and macrophages. Upon detection of its ligand, the activated C3aR signaling cascade results in degranulation, superoxide production, and chemotaxis.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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