PE Conjugated Anti-Hu CD118 (Clone: 12D3)
Figure 1: Separation of JAR cells stained using anti-human CD118 (12D3) PE antibody (concentration in sample 15 μg/ml, red-filled) from JAR cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 15 μg/ml, same as CD118 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of JAR cell suspension.
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 kappa |
| Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Concentration: 0.1 mg/ml Storage Buffer: Phosphate buffered saline (PBS), pH 7.4 |
| Storage condition : | Store at 2-8°C protected from light. Do not freeze. |
CD118 (LIFR alpha) is a type I transmembrane glycoprotein of the cytokine receptor family, which associates noncovalently with CD130 to form the functional high affinity LIF receptor, which also acts as an oncostatin M receptor. CD118 alone binds LIF with low affinity. A secreted form of CD118 results from alternative splicing, and may have inhibitory effect, as it also binds LIF, although with low affinity. CD118 is not expressed on lymphocytes, but it is widely expressed outside the immune system. Soluble CD118 level rises during pregnancy, in parallel with a drop in circulating LIF levels.
Specificity :The mouse monoclonal antibody 12D3 recognizes an extracellular epitope of CD118, a transmembrane glycoprotein, which associates with CD130 to form the functional high affinity LIF receptor.
Flow cytometry: Recommended dilution: 4-10 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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