Mouse Tumor Necrosis Factor-alpha (AF)

Source: Genetically modified E.coli.
Predicted MW: Monomer, 17.4 kDa (157 aa)
Tumor necrosis factor alpha (TNF-alpha) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-alpha signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-alpha functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Biological Activity was determined by Cytolysis of mouse L929 cells in the presence of Actinomycin D at <=100 pg/mL; >= 1.0 x 10^7 units/mg (typcial ED50 is < 20 pg/mL). Centrifuge vial before opening, Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Secreted
Post transnational modification:	O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
BioGrid:	204240. 12 interactions.

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