Mouse Monoclonal Antibody to V5 Tag (Clone : 4C12E11) (Discontinued)
Figure-1 : Western blot analysis of V5 Tag Antibody (Clone: 4C12E11) at 0.2 µg/ml on purified N-terminal of V5 tagged fusion proteins (50, 20, 10 & 5 ng), IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG was used as secondary Antibody.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG2a K |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Immunogen Information : | V5 tag peptide conjugated to KLH |
The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope. V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
ELISA: 0.05-0.1 µg/ml
Western blot: 0.2-1 µg/ml
FACS: 2-4 µg for 1 x 106 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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