Mouse Monoclonal Antibody to SUMO1 (Clone: 66AT1273.94)(Discontinued)

Name: Mouse Monoclonal Antibody to SUMO1 (Clone: 66AT1273.94)(Discontinued)
Brand: Abeomics
SKU: 10-6519
Price: 472.0000 USD
Availability: InStock
Rating: 5 (10 reviews)

Figure 1: Staining of SUMO1 antibody (10-6519) in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Mouse Monoclonal Antibody to SUMO1 (Clone: 66AT1273.94)(Discontinued)

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Figure 1: Staining of SUMO1 antibody (10-6519) in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Figure 2: Staining of SUMO1 antibody (10-6519) in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Figure 3: Overlay histogram showing Jurkat cells stained with (10-6519). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody with 1:25 dilution for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/2

Figure 4: All lanes : Anti-SUMO1 Antibody at 1:4000 dilution with Lane 1: HL-60 whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: Jurkat whole cell lysates/proteins at 20 Î¼g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa.

Figure 5: The SUMO1 monoclonal antibody (10-6519) is used in Western blot to detect SUMO1 in HL60 cell lysate.

Product code: 10-6519

Clone name :	66AT1273.94
Clonality :	Monoclonal
Application :	IHC-P, FACS, WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	400 µl
Isotype :	Mouse IgG1
Purification :	Protein G Chromatography
Content :	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage condition :	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles.


Gene :	SUMO1
Gene ID :	7341
Uniprot ID :	P63165
Alternative Name :	Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1, SUMO1, SMT3C, SMT3H3, UBL1

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.

IHC-P~1:25|| FACS~1:25|| WB~1:100~4000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus membrane, Nucleus speckle, Cytoplasm, Nucleus, Cell membrane, Nucleus
Post transnational modification:	Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
BioGrid:	113188. 169 interactions.