Mouse Monoclonal Antibody to Phosphotyrosine (Clone : 5E10E1)(Discontinued)
Figure-1 : Western blot analysis of Phosphotyrosine Antibody (Clone: 5E10E1) on EGF-stimulated A431 cell lysates and untreated A431 cell lysates at 1 µg/ml. IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG was used as secondary Antibody.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, k |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Immunogen Information : | Phosphotyrosine containing carrier protein |
Phosphotyrosine is a tyrosine residue covalently bound to a phosphate via its hydroxyl group. A tyrosine kinase catalyzes the transfer of a phosphate from ATP to a tyrosine residue on a protein substrate. Tyrosine phosphorylation plays a key role in intracellular signaling and cancer development. Many tyrosine kinases are drug targets for different forms of cancer. Phosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuable tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery. Phosphotyrosine Antibody (5E10), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with phosphotyrosine containing carrier protein.
Western Blot: 1-2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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