Mouse Monoclonal Antibody to LC3 (APG8)(Clone: 166AT1234)(Discontinued)

Figure 1: Anti-MAP1LC3A Antibody (10-6525) at 1:5000 dilution + recombinant protein at 20 Î¼g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa.

Mouse Monoclonal Antibody to LC3 (APG8)(Clone: 166AT1234)(Discontinued)

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Figure 1: Anti-MAP1LC3A Antibody (10-6525) at 1:5000 dilution + recombinant protein at 20 Î¼g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa.

Figure 2: All lanes : Anti-MAP1LC3A Antibody (10-6525) at 1:2000 dilution with Lane 1: human brain lysates, Lane 2: mouse brain lysates and Lane 3: rat brain lysates/proteins at 20 Î¼g per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa.

Figure 3: Immunofluorescent analysis of U251 cells, using LC3 Antibody (10-6525). U251 cells (right) were treated with Chloroquine (50 Î¼M,16h). LC3 Antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Figure 4: Immunofluorescent analysis of U251 cells, using LC3 Antibody (10-6525). U251 cells (right) were treated with Chloroquine (50 Î¼M,16h). LC3 Antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Figure 5: Fluorescent image of U251 cells stained with LC3 antibody (10-6525). U251 cells were treated with Chloroquine (50 Î¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 Î¼g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

$Figure 6 : Western blot analysis of anti-LC3 Mab (10-6525) at 8 Î¼g/ml with Lane 1: Y79 (soluble fraction of cell extract) and Lane 2: 293 transfected with human LC3 (whole cell extract).$

Figure 7: Western blot analysis of anti-LC3 Mab (10-6525) in Hela cell lysates, which were treated with rapamycin or bafilomycin overnight.

Product code: 10-6525

Clone name :	166AT1234
Clonality :	Monoclonal
Application :	WB, IF
Reactivity :	Rat, Mouse, Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	400 µl
Isotype :	Mouse IgG1
Purification :	Protein G Chromatography
Content :	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage condition :	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles.


Gene :	MAP1LC3A
Gene ID :	84557
Uniprot ID :	Q9H492
Alternative Name :	Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

WB~1:2000-5000|| IF~1:25-200

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Endomembrane system, Cytoplasmic vesicle, Cytoplasmic vesicle
Post transnational modification:	Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.
Tissue Specificity:	Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
BioGrid:	124137. 121 interactions.