Mouse Monoclonal Antibody to CD44 (Clone: Hermes-3)(Discontinued)

Figure 1: Staining of CD44 antibody (10-6555) in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Mouse Monoclonal Antibody to CD44 (Clone: Hermes-3)(Discontinued)

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Figure 1: Staining of CD44 antibody (10-6555) in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Figure 2: Staining of CD44 antibody (10-6555) in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Figure 3: Staining of CD44 antibody (10-6555) in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody

Figure 4: Staining of CD44 antibody (10-6555) in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Figure 5: All lanes : Anti-CD44 Antibody (10-6555) at 1:2000 dilution with Lane 1: Hela whole cell lysate and Lane 2: HUVEC whole cell lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa.

Figure 6: Immunohistochemistry analysis of CD44 antibody (10-6555) in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated

Figure 7: Confocal immunofluorescent analysis of CD44 antibody (10-6555) with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).

Product code: 10-6555

Clone name :	Hermes-3
Clonality :	Monoclonal
Application :	WB, IF
Reactivity :	Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	400 µl
Isotype :	Mouse IgG2a ,Kappa
Purification :	Protein G Chromatography
Content :	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage condition :	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles.


Gene :	CD44
Gene ID :	960
Uniprot ID :	P16070
Alternative Name :	CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

WB~1:2000|| IHC-P~1:10~50|| IF~1:10~50

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Cell projection
Post transnational modification:	Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Tissue Specificity:	Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
BioGrid:	107398. 132 interactions.