Mouse Monoclonal Antibody to CASP8 (C-term)(Clone: 550CT8.5.2)(Discontinued)

Name: Mouse Monoclonal Antibody to CASP8 (C-term)(Clone: 550CT8.5.2)(Discontinued)
Brand: Abeomics
SKU: 10-6584
Price: 472.0000 USD
Availability: InStock
Rating: 4 (10 reviews)

Figure 1: Overlay histogram showing Jurkat cells stained with CASP8 Antibody (10-6584) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1Î¼g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Mouse Monoclonal Antibody to CASP8 (C-term)(Clone: 550CT8.5.2)(Discontinued)

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Figure 1: Overlay histogram showing Jurkat cells stained with CASP8 Antibody (10-6584) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1Î¼g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Figure 2: All lanes : Anti-CASP8 Antibody (10-6584) at 1:2000 dilution with Lane 1: HepG2 whole cell lysate, Lane 2: Jurkat whole cell lysate and Lane 3: HL-60 whole cell lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa.

Product code: 10-6584

Clone name :	550CT8.5.2
Clonality :	monoclonal
Application :	FACS, WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	200 µl
Isotype :	Mouse IgG1
Purification :	Protein G Chromatography
Content :	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage condition :	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles.


Gene :	CASP8
Gene ID :	841
Uniprot ID :	Q14790
Alternative Name :	Caspase-8, CASP-8, 3.4.22.61, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10, CASP8, MCH5
Immunogen Information :	Synthesized Peptide

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

FACS~1:25|| WB~1:2000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
Tissue Specificity:	Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
BioGrid:	107291. 153 interactions.