Mouse Monoclonal Antibody to ALDH1A1 (Clone: 152CT1.2.2)(Discontinued)
Figure 1: Overlay histogram showing A549 cells stained with ALDH1A1 Antibody (10-6536) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Shipping Info:
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Format : | Purified |
Amount : | 400 µl |
Isotype : | Mouse IgG1 |
Purification : | Protein G Chromatography |
Content : | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
Storage condition : | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles. |
FACS~1:25|| IF~ 1:10~100|| WB~1:500~1000|| IHC-P~1:50~100
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm |
Post transnational modification: | The N-terminus is blocked most probably by acetylation. |
BioGrid: | 106718. 6 interactions. |
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