Mouse Migration Inhibitory Factor (AF)

Product code: 32-12245

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  •   25 µg

  •  100 µg

  • $430.00 

  • $757.00 

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Amount : 100 µg
Purification : Reducing and Non-Reducing SDS PAGE at >= 95%
Content : Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Sterile water at 0.1 mg/mL
Storage condition : Store at -20°C
AA sequence : MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTNDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA
Gene : Mif
Gene ID : 17319
Uniprot ID : P34884
Alternative Name : Macrophage migration inhibitory factor, Delayed early response protein 6, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase
Source: Genetically modified E.coli.
Predicted MW: Monomer, 12.5 kDa (115 aa)
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity.  MIF promotes fibroblast migration by inducing interleukin 1 (IL-1), interleukin 8 (IL-8), and matrix metalloproteinase (MMP) expression.  In interferon-gamma-activated macrophages, MIF stimulates nitric oxide (NO) production and tumor necrosis factor alpha (TNF-alpha) secretion. 

Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Centrifuge vial before opening, Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.   

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Secreted, Cytoplasm
BioGrid: 201418. 1 interactions.
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