Mouse IL-6 (Interleukin 6) Pre-Coated ELISA Kit

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-6 can be calculated. 

This immunoassay kit allows for the in vitro quantitative determination of mouse IL-6 concentrations in serum, plasma and other biological fluids.

Detection Range: 15.6 – 1000 pg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.