Mouse IL-6 (Interleukin 6) Pre-Coated ELISA Kit
Figure-1: Mouse IL-6 Standard Curve.
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| Amount : | 1 X 96 Tests |
| Content : | 1 X 96 well Format (96 tests) |
| Storage condition : | Please refer to the Manual |
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-6 can be calculated.
This immunoassay kit allows for the in vitro quantitative determination of mouse IL-6 concentrations in serum, plasma and other biological fluids.
Detection Range: 15.6 – 1000 pg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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