Mouse IL-1α (Interleukin-1 Alpha) Pre-Coated ELISA Kit

Product code: 90-2254

Application : ELISA
Reactivity : Mouse

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1 X 96 Tests
$562.00 

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Amount : 1 X 96 Tests
Content : 1 X 96 well Format (96 tests)
Storage condition : Please refer to the Manual

This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

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This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-1a concentrations in serum, plasma and other biological fluids. 

Detection Range: 15.6– 1000 pg/ml

Sensitivity: < 9.4 pg / ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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