Mouse IgG1 Isotype Control (Clone: MOPC31C)

Fig-1: Cell surface flow analysis of Mouse IgG1 isotype control in Jurkat cell line using 2 µg/10^6 cells (Clone: MOPC31C). Black represents cell only; red represents Mouse IgG1 isotype control. Goat anti-mouse PE conjugate was used as secondary antibody.

Mouse IgG1 Isotype Control (Clone: MOPC31C)

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Product code: 10-101

Clone name :	MOPC31C
Clonality :	Monoclonal
Application :	IHC, FACS
Reactivity :	Human

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Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1
Purification :	Affinity Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the mouse isotype control at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.

FACS: 0.25-2 µg/10^6 cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

1. Nayak TK, Mamidi P, Kumar A, Singh LP, Sahoo SS, Chattopadhyay S, Chattopadhyay S. Regulation of Viral Replication, Apoptosis and Pro-Inflammatory Responses by 17-AAG during Chikungunya Virus Infection in Macrophages. Viruses. 2017 Jan 6;9(1). pii: E3. doi: 10.3390/v9010003. PubMed PMID: 28067803; PubMed Central PMCID: PMC5294972.

2. Nayak TK, Mamidi P, Sahoo SS, Kumar PS, Mahish C, Chatterjee S, Subudhi BB, Chattopadhyay S, Chattopadhyay S. P38 and JNK Mitogen-Activated Protein Kinases Interact With Chikungunya Virus Non-structural Protein-2 and Regulate TNF Induction During Viral Infection in Macrophages. Front Immunol. 2019 Apr 12;10:786. doi: 10.3389/fimmu.2019.00786. eCollection 2019. PubMed PMID: 31031770; PubMed Central PMCID: PMC6473476.

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