Monoclonal Antibody to Vimentin (Clone: 2D1)

Name: Monoclonal Antibody to Vimentin (Clone: 2D1)
Brand: Abeomics
SKU: 34-1128
Price: 462.0000 USD
Availability: InStock
Rating: 4.9 (10 reviews)

Figure-1: Immunofluorescent analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin,(34-1128), dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP),(34-1046), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.

Monoclonal Antibody to Vimentin (Clone: 2D1)

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Figure-1: Immunofluorescent analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin,(34-1128), dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP),(34-1046), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.

Figure-2: Western blot analysis of different cell lysates using mouse mAb to vimentin,(34-1128), dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.

Product code: 34-1128

Clone name :	2D1
Clonality :	Monoclonal
Application :	WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG2a
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	VIM
Gene ID :	7431
Uniprot ID :	P08670
Immunogen Information :	Recombinant human vimentin expressed in and purified from E. coli.

WB: 1:10,000. IF/ICC and IHC: 1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cytoplasm, Nucleus matrix
Post transnational modification:	S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
BioGrid:	113272. 291 interactions.