Monoclonal Antibody to Vimentin (Clone: 2D1)
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin,(34-1128), dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP),(34-1046), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.
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Shipping Info:
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Mouse, IgG2a |
| Content : | Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:10,000. IF/ICC and IHC: 1:1,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm, Nucleus matrix |
| Post transnational modification: | S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| BioGrid: | 113272. 291 interactions. |
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