Monoclonal Antibody to Vimentin (Clone: 2A52)
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to vimentin,(34-1127), dilution 1:5,000 in green, and costained with rabbit pAb to glial fibrillary acidic protein (GFAP),(34-1042), dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. Fibroblastic, microglial and developing astrocytic cells contain only vimentin, and so appear green. Maturing astrocytic cells contain variable amounts of GFAP and vimentin, and so may appear red or yellow.
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Shipping Info:
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Format : | Purified |
Amount : | 100 µl |
Isotype : | Mouse, IgG1 |
Content : | Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:10,000. IF/ICC and IHC: 1:5,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm, Cytoplasm, Nucleus matrix |
Post transnational modification: | S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
BioGrid: | 113272. 291 interactions. |
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