Monoclonal Antibody to Vimentin (Clone: 2A52)

Name: Monoclonal Antibody to Vimentin (Clone: 2A52)
Brand: Abeomics
SKU: 34-1127
Price: 462.0000 USD
Availability: InStock
Rating: 4.3 (10 reviews)

Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to vimentin,(34-1127), dilution 1:5,000 in green, and costained with rabbit pAb to glial fibrillary acidic protein (GFAP),(34-1042), dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. Fibroblastic, microglial and developing astrocytic cells contain only vimentin, and so appear green. Maturing astrocytic cells contain variable amounts of GFAP and vimentin, and so may appear red or yellow.

Monoclonal Antibody to Vimentin (Clone: 2A52)

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Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to vimentin,(34-1127), dilution 1:5,000 in green, and costained with rabbit pAb to glial fibrillary acidic protein (GFAP),(34-1042), dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. Fibroblastic, microglial and developing astrocytic cells contain only vimentin, and so appear green. Maturing astrocytic cells contain variable amounts of GFAP and vimentin, and so may appear red or yellow.

Figure-2: Western blot analysis of cell and whole brain tissue lysates using mouse mAb to vimentin,(34-1127), dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).

Product code: 34-1127

Clone name :	2A52
Clonality :	Monoclonal
Application :	WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	VIM
Gene ID :	7431
Uniprot ID :	P08670
Immunogen Information :	Recombinant human vimentin expressed in and purified from E. coli.

WB: 1:10,000. IF/ICC and IHC: 1:5,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cytoplasm, Nucleus matrix
Post transnational modification:	S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
BioGrid:	113272. 291 interactions.