Monoclonal Antibody to TNF-alpha (Tumor Necrosis Factor alpha)(Clone : SPM543)

Product code: 36-1747

Clone name : SPM543
Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Rat, Mouse, Human

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100 µg
$520.00 

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]


Format : Purified
Amount : 100 µg
Isotype : Mouse IgM, kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : TNF
Gene ID : 7124
Uniprot ID : P01375
Alternative Name : TNF, TNFA, TNFSF2
Immunogen Information : A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjµgated to thyroglobulin

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Secreted
Post transnational modification: O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
BioGrid: 112979. 269 interactions.
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