Monoclonal Antibody to TLR9 (Clone: ABM1C51)

Fig-1: Immunohistochemical analysis of TLR9 in human stomach tissue using TLR9 antibody (Clone: ABM1C51) at 5 µg/ml.

Monoclonal Antibody to TLR9 (Clone: ABM1C51)

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Fig-1: Immunohistochemical analysis of TLR9 in human stomach tissue using TLR9 antibody (Clone: ABM1C51) at 5 µg/ml.

Fig-2: Western blot analysis of TLR9. Anti- TLR9 antibody (Clone: ABM1C51) was used at 2 µg/ml on (1) Raji and (2) EL4 lysates.

Fig-3: Intracellular flow analysis of TLR9 in human PBMC (Lymphocytes) using 0.5 µg/10^6 cells of TLR9 antibody (Clone: ABM1C51). Green represents isotype control; red represents anti-TLR9 antibody. Goat anti-mouse PE conjugate was used as secondary.

Fig-4: Intracellular flow analysis of TLR9 in Raji cells using 0.5 µg/10^6 cells of TLR9 antibody (Clone: ABM1C51). Green represents isotype control; red represents anti-TLR9 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.

Fig-5 : Immunohistochemical analysis of TLR9 in human Liver tissue using TLR9 antibody (Clone: ABM1C51) at 20 µg/ml.

Figure-6: Western blot analysis of TLR9. Anti- TLR9 antibody (Clone: ABM1C51) was used at 4 µg/ml on Daudi lysate.

Product code: 10-3003

Clone name :	ABM1C51
Clonality :	Monoclonal
Application :	IHC, FACS, WB
Reactivity :	Mouse, Human

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Order now and get it on Tuesday December 24, 2024

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Download TDS MSDS

Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1 Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	TLR9
Gene ID :	54106
Uniprot ID :	Q9NR96
Alternative Name :	TLR9, UNQ5798/PRO19605
Immunogen Information :	A partial length recombinant TLR9 protein (amino acids 100-290) was used as the immunogen for the antibody.

TLR9, a member of toll-like receptor family are central to the innate immunity by identifying pathogen associated molecular patterns (PAMPs). TLR9 identify unmethylated CpG dinucleotides present in bacterial DNA leading to NF-kB activation.

Western blot analysis: 2-4 µg/ml, FACS analysis: 0.5 µg/10^6 cells, Immunohistochemical analysis: 5 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Veleeparambil M, Poddar D, Abdulkhalek S, Kessler PM, Yamashita M, Chattopadhyay S, Sen GC. Constitutively Bound EGFR-Mediated Tyrosine Phosphorylation of TLR9 Is Required for Its Ability To Signal. J Immunol. 2018 Apr 15;200(8):2809-2818. doi: 10.4049/jimmunol.1700691. Epub 2018 Mar 12. PubMed PMID: 29531172; PubMed Central PMCID: PMC5893352.

Subcellular location:	Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle
Post transnational modification:	Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling.
Tissue Specificity:	Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
BioGrid:	119902. 20 interactions.