Monoclonal Antibody to RunX3 (Clone: ABM28D1)

Figure-1: Western blot analysis of RunX3. Anti- RunX3 antibody (Clone: ABM28D1) was used at 2 µg/ml on (1) HL-60 and (2) Jurkat lysates.

Monoclonal Antibody to RunX3 (Clone: ABM28D1)

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Figure-1: Western blot analysis of RunX3. Anti- RunX3 antibody (Clone: ABM28D1) was used at 2 µg/ml on (1) HL-60 and (2) Jurkat lysates.

Figure-2:Intracellular flowcytometry analysis of RunX3 on Raji cells using 0.5 µg/10^6 Cells of Anti-RunX3 (Clone: ABM28D1). Green represent isotype control and red represent Anti RunX3 antibody (Abeomics 10-4053). Goat anti mouse PE conjugated was used as the secondary antibody.

Figure-3:Intracellular flowcytometry analysis of RunX3 on Ramos cells using 0.5 µg/10^6 Cells of Anti-RunX3 (Clone: ABM28D1). Green represent isotype control and red represent Anti RunX3 antibody (Abeomics 10-4053). Goat anti mouse PE conjugated was used as the secondary antibody.

Product code: 10-4053

Clone name :	ABM28D1
Clonality :	Monoclonal
Application :	FACS, WB
Reactivity :	Human

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Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG2b Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	RUNX3
Gene ID :	864
Uniprot ID :	Q13761
Alternative Name :	RUNX3, AML2, CBFA3, PEBP2A3
Immunogen Information :	A partial length recombinant Runx3 protein (amino acids 100-320) was used as the immunogen for this antibody.

Runt-related transcription factor 3 (Runx3) forms a heterodimer of alpha and beta subunit and binds to the core of the promoter to regulate the transcription. It plays an important role in tumor suppressor and the gene is frequently deleted or transcriptionally silenced during tumor development. It has multiple isoforms coded by multiple transcript variances.

Western blot analysis: 2-4 µg/ml, Flowcytometry analysis: 0.5-1 µg/10^6 Cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus, Cytoplasm
Post transnational modification:	Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Tissue Specificity:	Expressed in gastric cancer tissues (at protein level).
BioGrid:	107312. 30 interactions.