Monoclonal Antibody to Peripherin (Clone: 8G2)

Figure-1: Immunofluorescent analysis of human neuroblastoma cell line SH-SY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with mouse mAb to peripherin,(34-1100), dilution 1:500, in red, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament (IF) subunit proteins is revealed by (34-1100) antibody in the perinuclear region in some SH-SY5Y cells and in all PC12 cells. Vimentin, a protein also in the Class III IF family, is detected in a subpopulation of SH-SY5Y cells which are negative for peripherin. PC12 cells express peripherin but not  vimentin.