Monoclonal Antibody to PAX8 (Clone:ABM5F33 )
Figure-1: Intracellular flow analysis of Pax8 in Raji cells using 0.5 µg/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 2-4 µg/ml, FACS: 0.5-1 µg/10^6
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus |
| Tissue Specificity: | Expressed in the excretory system, thyroid gland and Wilms tumors. |
| BioGrid: | 113604. 9 interactions. |
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