Monoclonal Antibody to Nop1p, Fibrillarin (Clone: 38F3)(Discontinued)
Figure 1: Staining of neuroblastoma SHSY-5Y cells with 34-1090 which binds to nucleoli (green). Cells are also stained in red with chicken polyclonal antibody to neurofilament NF-H 34-1080 in red. DNA is revealed with DAPI which binds DNA. Since nucleoli are found in the nucleus the punctate green fibrillarin stain is overlaid with blue, giving a pale whitish signal.
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Format : | T.C. Sup. |
Amount : | 500 µl |
Isotype : | Mouse, IgG1 |
Content : | Antibody is supplied as an aliquot of concentrated tissue culture supernatant. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Western blot: 1:500-1:1,000. ICC/IF and IHC: 1:1,000-1:5,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus |
Post transnational modification: | Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues. |
BioGrid: | 32040. 356 interactions. |
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