Monoclonal Antibody to NIFK (Clone: ABM29H6)
Fig-1: Western blot analysis of NIFK. Anti- NIFK antibody (Clone: ABM29H6) was used at 1 µg/ml on K562 lysate.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1 Kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
NIFK (Nucleolar protein interacting with the FHA domain of pKi-67) or MKI67 FHA domain-interacting nucleolar phosphoprotein is a 34 kDa protein localized within nucleolus of cell. NIFK has a potential RNA-binding domain which interacts with the forkhead-associated domain of the MKI67. NIFK plays a role in mitosis and cell cycle progression. Sequential phosphorylation on Thr-238, Thr-234 and Ser-230 enhances MKI67 binding. NIFK is highly expressed in brain and kidney tissues.
Western blot analysis: 1-2 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding. |
| BioGrid: | 124066. 151 interactions. |
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