Monoclonal Antibody to Neurofilament NF-M (Clone: 3H11)

Figure-1: Immunofluorescence analysis of adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M,(34-1085), dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, (34-1080), dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. (34-1085) antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody, which is primarily directed against the heavily phosphorylated axonal forms of this molecule, and so stains the network of neuronal axons only.