Monoclonal Antibody to Neurofilament NF-H (Clone: AH1)

Name: Monoclonal Antibody to Neurofilament NF-H (Clone: AH1)
Brand: Abeomics
SKU: 34-1075
Price: 462.0000 USD
Availability: InStock
Rating: 4.1 (10 reviews)

Figure-1: Immunohistological analysis of rat cerebellum section stained with mouse mAb to pNF-H,(34-1075), dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN,(34-1036), dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The (34-1075) antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.

Monoclonal Antibody to Neurofilament NF-H (Clone: AH1)

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Figure-1: Immunohistological analysis of rat cerebellum section stained with mouse mAb to pNF-H,(34-1075), dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN,(34-1036), dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The (34-1075) antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.

Figure-2: Western blot detection of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20µg/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (34-1075)) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.

Product code: 34-1075

Clone name :	AH1
Clonality :	Monoclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, Rat, Mouse, Cow, Pig, Horse

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Ascites
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of ascites fluid or affinity purified preparation at 1 mg/ml.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	NEFH
Gene ID :	4744
Uniprot ID :	P12036
Alternative Name :	200 kDa neurofilament protein, Neurofilament triplet H protein
Immunogen Information :	Native NF-H purified from bovine spinal cord

WB: 1:10,000. ICC/IF and IHC: 1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
BioGrid:	110819. 25 interactions.