Monoclonal Antibody to Neurofilament NF-H (Clone: 9B12)

Name: Monoclonal Antibody to Neurofilament NF-H (Clone: 9B12)
Brand: Abeomics
SKU: 34-1076
Price: 462.0000 USD
Availability: InStock
Rating: 4.9 (10 reviews)

Figure-1: Immunohistological analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, (34-1076), dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibody. The (34-1076) antibody is a robust marker of the axons of neuronal cells.

Monoclonal Antibody to Neurofilament NF-H (Clone: 9B12)

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Figure-1: Immunohistological analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, (34-1076), dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibody. The (34-1076) antibody is a robust marker of the axons of neuronal cells.

Figure-2: Western blot analysis of different tissue lysates using mouse mAb to NF-H,(34-1076), dilution 1:10,000 in green: [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.

Product code: 34-1076

Clone name :	9B12
Clonality :	Monoclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, Rat, Mouse

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Ascites
Amount :	100 µl
Isotype :	Mouse, IgG2b
Content :	Antibody is supplied as an aliquot of of 1 mg/ml of affinity purified antibody or ascites fluid.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	NEFH
Gene ID :	4744
Uniprot ID :	P12036
Alternative Name :	200 kDa neurofilament protein, Neurofilament triplet H protein
Immunogen Information :	Native NF-H purified from bovine spinal cord.

WB: 1:10,000. ICC/IF and IHC: 1:1,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
BioGrid:	110819. 25 interactions.