Monoclonal antibody to NDRG1 (Clone: ABM51C9 )
Fig:1- Expression analysis of NDRG1. Anti-NDRG1 antibody (Clone: ABM51C9) was tested at 4 µg/ml on (1) h Intestine, (2) h Ovary, (3) A431 and (4) HCT-116 lysates.
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG1 Kappa |
Purification : | Protein G Chromatography |
Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Western blot analysis: 4-6 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm, Cytoplasm, Nucleus, Cell membrane |
Post transnational modification: | Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent. |
Tissue Specificity: | Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression. |
BioGrid: | 115669. 111 interactions. |
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