Monoclonal Antibody to MyoD1 (Rhabdomyosarcoma Marker)(5.8A + MYD712)(Discontinued)

Product code: 36-1491

Clone name : 5.8A + MYD712
Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human, Mouse, Rat, Chicken

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : MYOD1
Gene ID : 4654
Uniprot ID : P15172
Alternative Name : MYOD1, BHLHC1, MYF3, MYOD
Immunogen Information : Recombinant mouse MyoD1 protein (5.8A); Recombinant human MyoD1 protein (MYD712)
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus
Post transnational modification: Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
BioGrid: 110737. 69 interactions.
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