Monoclonal Antibody to Myeloid-Related Proteins 14 (MRP14) (Macrophage Marker)(Clone : MRP14/840)(Discontinued)

Recognizes a protein of14kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This MAb reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Secreted, Cytoplasm, Cytoplasm, Cell membrane
Post transnational modification:	Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
BioGrid:	112188. 114 interactions.

There are currently no product reviews Write a review on this product!