Monoclonal Antibody to Mouse ICOSL (Clone: HK5.3)
Figure 1: Cell surface staying of splenocytes stimulated with ConA + mIL2 for 3 days. Green represents Rat isotope control, red represents Anti-mouse IcosL(10-7643). 0.2 µgantibodies were used. Goat anti-rat PE was used as secondary antibody.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rat IgG2a, Kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Flowcytometric Analysis: 0.2-0.4 µg/10^6 Cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane |
| Tissue Specificity: | Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the follicles), lower levels in many non-lymphoid tissues, such as brain, heart, kidney, liver, lung, skeletal muscle and testis. Present on freshly isolated splenic B-cells, T-cells, dendritic cells and macrophages. The expression of isoform 2 is restricted to heart, spleen and kidney. |
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