Monoclonal Antibody to Microtubule Associated Protein 2, MAP2A/B (Clone: 5H11)
Figure-1: Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to MAP2,(34-1066), dilution 1:5,000 in green, and costained with rabbit pAb to α-internexin,(34-1055), dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The (34-1066) antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the α-internexin antibody selectively stains axons and dendrites of neuronal cells.
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Mouse, IgG1 |
| Content : | Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:10,000. IF/ICC and IHC: 1:1,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cell projection |
| Post transnational modification: | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). |
| BioGrid: | 110305. 48 interactions. |
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