Monoclonal Antibody to Microtubule Associated Protein 2, MAP2A/B (Clone: 5H11)

Name: Monoclonal Antibody to Microtubule Associated Protein 2, MAP2A/B (Clone: 5H11)
Brand: Abeomics
SKU: 34-1066
Price: 462.0000 USD
Availability: InStock
Rating: 4.3 (10 reviews)

Figure-1: Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to MAP2,(34-1066), dilution 1:5,000 in green, and costained with rabbit pAb to Î±-internexin,(34-1055), dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The (34-1066) antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the Î±-internexin antibody selectively stains axons and dendrites of neuronal cells.

Monoclonal Antibody to Microtubule Associated Protein 2, MAP2A/B (Clone: 5H11)

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Figure-1: Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to MAP2,(34-1066), dilution 1:5,000 in green, and costained with rabbit pAb to Î±-internexin,(34-1055), dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The (34-1066) antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the Î±-internexin antibody selectively stains axons and dendrites of neuronal cells.

Figure-2: Western blot analysis of different tissue lysates using mouse mAb to MAP2A/B,(34-1066), dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in adult brain particularly in cortical regions, but is a more minor component of spinal cord and almost absent from the embryonic brain sample. Note that since the epitope for this antibody in within the projection domain found only in MAP2A and MAP2B, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.

Product code: 34-1066

Clone name :	5H11
Clonality :	Monoclonal
Application :	WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	MAP2
Gene ID :	4133
Uniprot ID :	P11137
Immunogen Information :	Full length purified bovine protein. Epitope maps to 1057-1507 of the human sequence.

WB: 1:10,000. IF/ICC and IHC: 1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cell projection
Post transnational modification:	Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
BioGrid:	110305. 48 interactions.