Monoclonal Antibody to mFolate Receptor 4 (Clone: TH6)
Figure-1: Cell surface flowcytometric staining of mFR4 (Clone: TH6) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mFR4 using 0.5 µg/10^6. Goat anti-Rat PE secondary antibody (ABEOMICS) was used.
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Rat IgG2b Kappa |
Purification : | Protein G Chromatography |
Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
FACS: 0.5-1 µg/10^6
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cell membrane |
Post transnational modification: | The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm (PubMed:24739963). |
Tissue Specificity: | Widely expressed with higher expression in thymus, spleen and lung. Present at the cell surface of unfertilized oocytes, while it is barely detectable 30 to 40 minutes after fertilization (at protein level) (PubMed:24739963). |
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