Monoclonal Antibody to IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker)(IG217 + IG266)
Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Monoclonal Antibody (IG217 + IG266)
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa + Mouse IgG2a, kappa |
| Purification : | Affinity Chromatography |
| Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | IGHG1 |
| Uniprot ID : | P01857 |
| Alternative Name : | IGHG1 |
| Immunogen Information : | Purified human IgG |
This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Flow Cytometry (1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Secreted, Cell membrane |
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