Monoclonal Antibody to Human Nuclear Antigen (HNA) (Human Cell Marker)(Clone : 235-1)-PE
Flow Cytometry of Human Nuclear Antigen on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).
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Format : | Purified |
Amount : | 0.5 ml |
Isotype : | Mouse IgG1, kappa |
Content : | PE conjugated HNA , 0.5 ml at 100ug/ml in 10mM PBS with 0.05% BSA and 0.05% azide. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. |
Immunogen Information : | Nuclei of human myeloid leukemia biopsy cells |
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100 for 30 minutes at RT);
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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