Monoclonal Antibody to Heparan Sulfate Proteoglycan (Large) / Perlecan(Clone : SPM255)
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Heparan Sulfate Monoclonal Antibody (SPM255).
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Rat IgG2a, kappa |
Purification : | Affinity Chromatography |
Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Secreted |
Post transnational modification: | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Perlecan contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. |
Tissue Specificity: | Found in the basement membranes. |
BioGrid: | 109571. 35 interactions. |
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