Monoclonal Antibody to GAP43 (Clone: 5E8)

Name: Monoclonal Antibody to GAP43 (Clone: 5E8)
Brand: Abeomics
SKU: 34-1040
Price: 462.0000 USD
Availability: InStock
Rating: 4.2 (10 reviews)

Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43,(34-1040), dilution 1:1,000, in red, and costained with chicken pAb to GFAP,(34-1046), dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while the GFAP antibody stains astroglial cells.

Monoclonal Antibody to GAP43 (Clone: 5E8)

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Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43,(34-1040), dilution 1:1,000, in red, and costained with chicken pAb to GFAP,(34-1046), dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while the GFAP antibody stains astroglial cells.

Figure-2: Western blot analysis of tissue and cell lysates using mouse mAb to GAP43,(34-1040), dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at 43kDa corresponds to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin. Note that the protein from SH-SY5Y cells, the only line of human origin tested here, has a slightly lower SDS-PAGE than the rodent proteins.

Product code: 34-1040

Clone name :	5E8
Clonality :	Monoclonal
Application :	WB
Reactivity :	Human

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	GAP43
Gene ID :	2596
Uniprot ID :	P17677
Alternative Name :	Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46
Immunogen Information :	Â Recombinant full- length Human GAP43

WB: 1:1,000-5,000 IF/ICC and IHC: 1:1,000-5,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Cell projection, Cell junction, Cell projection
Post transnational modification:	Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
BioGrid:	108867. 27 interactions.