Monoclonal Antibody to Estrogen Receptor (Marker of Estrogen Dependence)(Clone : ER505)(Discontinued)
Western Blot of Estrogen Receptor in MCF-7Lysate using Estrogen Receptor Monoclonal Antibody (ER505).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1 Kappa |
| Purification : | Affinity Chromatography |
| Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This MAb is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.
Western Blot (0.5-1µg/ml for 2 hours at Room Temp); Immunohistology (Formalin-fixed) (1-2µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Golgi apparatus, Cell membrane |
| Post transnational modification: | Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor. |
| Tissue Specificity: | Widely expressed. Isoform 3 is not expressed in the pituitary gland. |
| BioGrid: | 108403. 738 interactions. |
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