Monoclonal Antibody to DNMT3a (Clone: ABM13G4 )

Fig-1: Western blot analysis of DNMT3a. Anti-DNMT3a antibody (Clone: ABM13G4) was used at 2 µg/ml on h heart lysate.

Monoclonal Antibody to DNMT3a (Clone: ABM13G4 )

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Fig-1: Western blot analysis of DNMT3a. Anti-DNMT3a antibody (Clone: ABM13G4) was used at 2 µg/ml on h heart lysate.

Fig-2: Intracellular flow cytometric analysis of Dnmt3a on HEK293 cells using 0.5 µg/10^6 cells of antibody (Clone: ABM13G4). Green represents isotype control; red represents anti-Dnmt3a antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).

Product code: 10-7003

Clone name :	ABM13G4
Clonality :	Monoclonal
Application :	FACS, WB
Reactivity :	Human

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Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1, Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	DNMT3A
Gene ID :	1788
Uniprot ID :	Q9Y6K1
Alternative Name :	DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA
Immunogen Information :	A partial length recombinant protein from DNMT3a was used as the immunogen for this antibody.

Dnmt3a belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. Dnmt3a together with other methyltransferases conducts de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring. Dnmt3a also directs a metabolic program by repressing key genes to enable the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been found in several cancers including AML (Acute Myeloid Leukemia).

WB: 2-4 µg/ml, FACS: 0.5-1 µg/10^6

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus, Cytoplasm
Post transnational modification:	Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Tissue Specificity:	Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
BioGrid:	108125. 53 interactions.