Monoclonal Antibody to DJ1 (Clone: 4H4)

Name: Monoclonal Antibody to DJ1 (Clone: 4H4)
Brand: Abeomics
SKU: 34-1027
Price: 462.0000 USD
Availability: InStock
Rating: 4.6 (10 reviews)

Figure-1: Immunoflourescent analysis of HeLa cells stained with mouse mAb against DJ1,(34-1027), dilution 1:1,000 in green, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000, in red. The (34-1027) antibody reveals strong cytoplasmic staining for DJ1 protein, while the(34-1126) antibody stains cytoplasmic intermediate filaments.

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Figure-1: Immunoflourescent analysis of HeLa cells stained with mouse mAb against DJ1,(34-1027), dilution 1:1,000 in green, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000, in red. The (34-1027) antibody reveals strong cytoplasmic staining for DJ1 protein, while the(34-1126) antibody stains cytoplasmic intermediate filaments.

Figure-2: Western blot analysis of whole brain and cell lysates using mouse mAb against DJ1,(34-1027), dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, (34-1126), dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.

Product code: 34-1027

Clone name :	4H4
Clonality :	Monoclonal
Application :	WB, ICC/IF, IHC
Reactivity :	Human, Cow, no reactivity with Rat or Mouse

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of purified antibody.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	PARK7
Gene ID :	11315
Uniprot ID :	Q99497
Alternative Name :	Oncogene DJ1, Parkinson disease protein 7
Immunogen Information :	Full length recombinant human DJ-1 expressed in and purified from E. coli.

WB: 1:5,000. IF/IHC: 1:1,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion
Post transnational modification:	Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
Tissue Specificity:	Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Expressed by pancreatic islets at higher levels than surrounding exocrine tissues (PubMed:22611253).
BioGrid:	116446. 169 interactions.