Monoclonal Antibody to Cytokeratin, Basic (Type II or HMW) (Epithelial Marker)(Clone : 34BE12)

Product code: 36-11091

Clone name : 34BE12
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human, Mouse, Rat

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100 µg
$520.00 

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]


Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1, kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : KRT76
Gene ID : 51350
Uniprot ID : Q01546
Alternative Name : KRT76, KRT2B, KRT2P
Immunogen Information : Solubilized keRatin extract from human stRatum corneum
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

1. Zhang, Y., Tang, C., Span, P. N., Rowan, A. E., Aalders, T. W., Schalken, J. A., Adema, G. J., Kouwer, P. H. J., Zegers, M. M. P., Ansems, M., Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation. Adv. Sci. 2020, 2001797. https://doi.org/10.1002/advs.202001797

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