Monoclonal Antibody to Cytokeratin 14 (KRT14) (Squamous Cell Marker)(Clone : SPM263)
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG3, kappa |
Purification : | Affinity Chromatography |
Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm, Nucleus |
Post transnational modification: | Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
Tissue Specificity: | Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. |
BioGrid: | 110059. 43 interactions. |
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