Monoclonal Antibody to Cytokeratin 10/13(Clone : SPM262)

Product code: 36-11008

Clone name : SPM262
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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100 µg
$520.00 

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG2a, kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : KRT10
Gene ID : 3858
Uniprot ID : P13645
Alternative Name : KRT10, KPP
Immunogen Information : Cytoskeletal prepaRation extracted from human ectocervical epithelium
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Secreted
Tissue Specificity: Seen in all suprabasal cell layers including stratum corneum.
BioGrid: 110056. 73 interactions.
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