Monoclonal antibody to CD16 (Clone: B73.1)
Figure 1: Cell surface staining of PBMC using anti-CD16, clone B73.1 antibody (10-4206). Green histogram: Isotype control. Red histogram: anti-CD16 antibody. Goat anti-mouse PE was used as secondary antibody. 0.5 µg antibody was used.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1k |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
CD16, also known as low affinity IgG receptor III (FcγRIII), is expressed as two distinct forms, referred to as CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein whose extracellular domain is over 95% homologous to that of CD16a, and is expressed specifically on neutrophils. CD16 binds to aggregated IgG or IgG-antigen complex, which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
FACS, IF
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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