Monoclonal Antibody to Carcinoembryonic Antigen (CEA) / CD66(COL-1 + CEA31 + C66/261)
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CEA Monoclonal Antibody (COL-1+CEA31+C66/261)
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse/IgG2a (COL-1) + Mouse/IgG1 (CEA31 and C66/261) |
Purification : | Affinity Chromatography |
Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cell membrane, Apical cell membrane, Cell surface |
Post transnational modification: | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
Tissue Specificity: | Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. |
BioGrid: | 107478. 6 interactions. |
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