Monoclonal Antibody to Calbindin(Clone: 5A9)

Figure-1: Immunofluorescent analysis of rat brain cerebellum section stained with mouse mAb to calbindin,(34-1019), dilution 1:2,000, in red, and costained with rabbit pAb to GFAP,(34-1042), dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The (34-1019) calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.