Monoclonal Antibody to Biotin (Vitamin B7 or Vitamin H)(Clone : Hyb-8)

Product code: 36-11017

Clone name : Hyb-8
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : All species

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100 µg
$520.00 

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1, kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information : Biotinylated sheep immunoglobulin
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Confocal Microscopy; In situ Nucleic Acid Hybridization; Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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