Monoclonal Antibody to Bcl-6 (Follicular Lymphoma Marker)(Clone : BCL6/850)(Discontinued)
Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with bcl-6 Monoclonal Antibody (BCL6/850).
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG1 Kappa |
Purification : | Affinity Chromatography |
Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Immunohistology (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus |
Post transnational modification: | Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways. |
Tissue Specificity: | Expressed in germinal center T- and B-cells and in primary immature dendritic cells. |
BioGrid: | 107076. 119 interactions. |
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