Monoclonal Antibody to Aurora B Kinase (Clone: 6G2)

Name: Monoclonal Antibody to Aurora B Kinase (Clone: 6G2)
Brand: Abeomics
SKU: 34-1017
Price: 462.0000 USD
Availability: InStock
Rating: 4.9 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to aurora B kinase,(34-1017), dilution 1:1,000 in red, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. (34-1017) antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.

Monoclonal Antibody to Aurora B Kinase (Clone: 6G2)

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Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to aurora B kinase,(34-1017), dilution 1:1,000 in red, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. (34-1017) antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.

Figure-2: Western blot analysis of different cell lysates and recombinant protein solutions using mouse mAb to aurora B,(34-1017). Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Human recombinant proteins aurora A, B, and C as indicated. This antibody binds specifically to aurora B.

Product code: 34-1017

Clone name :	6G2
Clonality :	Monoclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, Horse, Dog

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Download TDS MSDS

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	AURKB
Gene ID :	9212
Uniprot ID :	Q96GD4
Alternative Name :	Aurora 1, AIM-1, Aurora- and IPL1-like midbody-associated protein 1, ARK-2, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B
Immunogen Information :	Full length recombinant human Aurora B protein expressed in and purified from E. coli.

WB: 1:1,000. IF/ICC or IHC: 1:1,000-1:2,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus, Chromosome, Chromosome, Cytoplasm, Midbody
Post transnational modification:	Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Tissue Specificity:	High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
BioGrid:	114646. 170 interactions.