Monoclonal Antibody to Aurora B Kinase (Clone: 6G2)
Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to aurora B kinase,(34-1017), dilution 1:1,000 in red, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. (34-1017) antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Mouse, IgG1 |
| Content : | Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | AURKB |
| Gene ID : | 9212 |
| Uniprot ID : | Q96GD4 |
| Alternative Name : | Aurora 1, AIM-1, Aurora- and IPL1-like midbody-associated protein 1, ARK-2, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B |
| Immunogen Information : | Full length recombinant human Aurora B protein expressed in and purified from E. coli. |
WB: 1:1,000. IF/ICC or IHC: 1:1,000-1:2,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome, Chromosome, Cytoplasm, Midbody |
| Post transnational modification: | Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. |
| Tissue Specificity: | High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. |
| BioGrid: | 114646. 170 interactions. |
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